My note on paper: A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95
Paper: A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95 (doi: 10.1016/j.yexcr.2008.01.013) Abstract; Facts; -RNF43 is important for colorectal cancer -Growth promoting factor Gap; -Molecular mechanism of how rnf43 promote growth is unk Method; -Biochemically and intracellularly characterize rnf43 Result; -Rnf43 is in the er and nuclear envelope -rnf43 coexists with emerin (inner nuclear membrane protein) -rnf43 has autoubiquitination activity -rnf43 can interact with hap95 (chromatin associated protein) -rnf43 ubiquitinylate hap95 but only in some cell lines --- -using available database (bioexpress), rnf43 is highly expressed in colon adenoma and colon adenocarcinoma -rnf43 is overexpressed in colorectal tumor -evident showed when this gene is knockdown exerts growth inhibition --- Cell lines; HeLa COS-7 --- this paper contradict to the previous report that RNF43 is secretory pr...