Note for Increased Ratio of Targeted to Random Integration after Transfection of Chicken B Cell Lines
Note for: Increased Ratio of Targeted to Random Integration after Transfection of Chicken B Cell Lines (doi: 10.1016/0092-8674(91)90581-I ) Viral infection cell line; 1.avian leukosis virus-induced chicken B cell line 2. 4 cell line were used to check the targeted integration but only B-cell had the highest rate of targeted integration compare to non-B cell Factor contributes to the ratio of targeted integration/random (mammalian); 1.length of homology - the longer is better 2.transcription rate of target loci normal rate - 1/100-1/10,000 (in mammalian genome) There were the attempts to increase the rate of targeted in mammalian - modified the input DNA or synchronize the cells Main point of this paper; 1.Higher ratio of targeted integration in DT40 vs mammalian 2.Compare with other types of chicken cell lines, B-cell lines had a higher frequency of targeted integration. 3.Higher ratio of targeted integration in DT40 does not rely on the transcripti...