Note: Mutant cells defective in DNA repair pathways provide a sensitive high-throughput assay for genotoxicity
Note: Mutant cells defective in DNA repair pathways provide a sensitive high-throughput assay for genotoxicity DNA repair during replication (S-phase) 1. TLS 2. HR 3. FA DNA repair throughout cell cycle; 1. base excision 2. nucleotide excision 3. NHEJ In this paper, the panel that they used; 1. DNA damage checkpoint - ATM 2. HR - Rad54, XRCC3, and UBC13 3. NHEJ - Ku70 4. ICL - FANCC 5. TLS - Rev3 6. NER - XPA and XPG 7. BER - DNA pol-beta My understanding in this paper - they relied on one particular lesion will be majorly used by one particular pw (how much fold should be different then bc DNA repair has the redundancy?). Good points for DT40 again (in year 2010) - 1.High targeted integration 2.Stable karyotype/phenotype even for the long subculture 3.Short doubling time Still, it is chicken - some genes could not be conducted though DNA repair pws have been said to be conserved throughout evolution. 1. Genetic manipulation in mammalian cells - diffi...