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Note: COSMIC-3D provides structural perspectives on cancer genetics for drug discovery (doi: 10.1038/s41588-018-0214-9) • COSMIC-3D is designed to enable greater understanding of the functional impact of mutations, generate new hypotheses on which mutations are cancer drivers and provide new opportunities for addressing these mutations pharmaceutically. • genetics, structural proteomics and drug development can be best described as ‘mutation-guided drug design’. • there remains much scope for expansion of the repertoire of precision therapeutic drugs targeting these mutations. • Both cancer genomic and protein structural data sources are individually great assets for scientists working in the field of oncology drug discovery. • COSMIC-3D currently maps cancer variant data for nearly 9,300 genes to nearly 37,000 protein structures, covering 390 key genes from the Cancer Gene Census. • The structure viewer displays the protein structure and small-molecule ligands, ...

A brief overview of how to get the 3D protein structure. 1. gene of interest 2. cloning it 3. expressing it 4. purify it 5. crystallize it - not really depend on protein size, it is the matter of luck, patient, and good observations. Three methods; microbatch -- good for screening (sample and precipitant are mixed and covered by the paraffin oil), vapor diffusion -- hanging drop (using the exchange between precipitant and reservoir), liquid diffusion -- hard to implement To solve with Synchrotron - the crystal should be in the range between 20-200 micrometers. Way to differentiate the protein crystal from the salt; staining with Coomassie after running the SDS-PAGE or directly checking from the diffraction - salt and protein give a very different diffraction pattern. 6. X-ray diffraction--phase--electron density map obtain--fitting--building the model! 7. Analyze data 8. Submit the 3D information at PDB Protein crystallography really depends on luck and it is the fine pie...