Note: Characterization of environmental chemicals with potential for DNA damage using isogenic DNA repair-deficient chicken DT40 cell lines
Note for: Characterization of environmental chemicals with potential for DNA damage using isogenic DNA repair-deficient chicken DT40 cell lines (doi: 10.1002/em.20656) US Tox21 program -- evaluate the detection of genotoxic cpds. This study focused on cytotoxicity by using DT40 with deficient in DNA repair pathway. Using 7-isogenic DNA repair-deficient pw cell line to identify the types of DNA damage. Criteria to judge; 1.clastogenicity (ability to break the DNA) in mutant and WT 2.ability to induce gH2Ax positive foci by melphalan 3.72-hr viability through the liquid assay 4.using more DNA repair deficient cell lines to clearly identify the damage induced 5.involvement of ROS in induction of DNA damage At the end, it proposed that DT40 and theirs DNA-repaired deficient lines are useful tool to detect 1.genotoxic cmp. 2.identify the nature of DNA damage of the challenges 3.analyzing mechanism of mutagenesis Rational; Because of the number of chemica...