Note for: Systematic E2 screening reveals a UBE2D-RNF138-CtIP axis promoting DNA repair
Note for: Systematic E2 screening reveals a UBE2D-RNF138-CtIP axis promoting DNA repair (doi: 10.1038/ncb3260) Very nice piece of work, I would say. It reflects the complexity of living thing is, especially, post-translational modification processes which control the cellular function through adding the small molecule namely ubiquitin to certain protein. Unrepaired DSB -- genome instability, tumorigenesis, neurodegeneration or premature ageing. The team used UBE2Ds to screen for the binding partner during the DSB responses (using IR in this case). This study found RNF138 was the binding partner for UBE2Ds during the DSB responses. RNF138 promotes CtIF ubiquitination. Key to initiating HR is DNA-end resection promoted by CtIP (RBBP8) recruitment to DSB sites, yielding single-stranded DNA (ssDNA) that is rapidly bound by RPA and subsequently replaced by RAD51, leading to strand invasion and ensuing HR processes. Gap -- how CtIP and early HR events are regulated, ho...