Note: Measure DNA repair_2014
Note: Measure DNA repair_2009 doi: 10.1080/13102818.2009.10817632 เป็นเปเปอร์ที่มี typo พอสมควร DNA mutations can cause diseases - thus understanding how cell repairs DNA is important. The ability to repair DNA to return to homeostasis state; Repair capacity Repair rate Repair specificity ------ Content Base sequences are kept securely by the backbone of phosphodiester bonds and N-glycosidic bonds -- very stable at the physiological condition Base stacking -- save the base from exposing to environment Any modification of the chemical structure of DNA -- DNA damages DNA damage Halt transcription Cause mutation during replication SSB Phosphodiester bond is being hydrolyzed -- leaving 5’ end phosphorylated and 3’ end deoxyribosyl moiety with 3’OH group SSB forms from the digestion of endonuclease Damaged base must be removed from phosphodiester backbone -- SSB forms Pyrimidine dimers UV light (260 nM) -- DNA expose -- causing photoproducts Two major types of photoproducts Cyclobutane pyri...