My note on paper: A novel Rad18 function involved in protection of the vertebrate genome after exposure to camptothecin
Paper: A novel Rad18 function involved in protection of the vertebrate genome after exposure to camptothecin (10.1016/j.dnarep.2006.05.045) S. cerevisiae; Rad18 function in PRR consist of 1.error-free damage bypass (Rad30 -Poln) 2.error-prone damage bypass (Rev3/7 -Pole) DT40; RAD18(-/-) cell 1. hypersensitivity to CPT but not RAD30(-/-)+REV3(-/-) 2. higher levels of H2Ax phosphorylation 3. higher in chromosomal aberration; gaps and breaks (after exposure to CPT) 4. no function in elongation without stimulant; but with CPT it showed more break In conclusion; this paper showed the role of RAD18 in replication fork when there is the lesion generate by CPT Context: CPT inhibit topoisomerase-I which is required for unsolve secondary structure during DNA replication Inhibition of CPT can cause the db strand break during replication, therefore, initiate the ATR/ATM dependent phosphorylation of H2Ax. There is evident showed DSB generated by CPT - being repair by HR. I...