Note for: Werner and Bloom helicases are involved in DNA repair in a complementary fashion
Note for: Werner and Bloom helicases are involved in DNA repair in a complementary fashion (doi: https://doi.org/10.1038/sj.onc.1205143 ) Werner syndrome; 1. rare autosomal recessive disorder 2. premature senescence Bloom syndrome; 1. short stature 2. immunodeficiency 3. rare autosomal recessive common for these two; 1. chromosomal instability-->tumorigenesis WRN and BLM-->closely relate to RecQ helicase WRN and BLM - involve in DNA repair in a complementary fashion. Both BLM and WRN; 1. contain 7 signature motifs conserved in DNA/RNA helicases 2. BLM shows most similarity to RecQ subfamily of DNA helicases Recombinant WRN and BLM; 1. unwind duplex DNA in 3-5 direction with 3' overhang tail 2. both interact with RPA to facilitate the unwinding WRN has the exonuclease activity (N-terminal) which functions as proofreading during the DNA synthesis. BLM and WRN show very different profiles of sensitivity toward DNA da...