SIM5 (Structured Illumination Microscopy) - Demonstrated by ZEISS
Note from the demo: SIM ZEISS_Demonstration at Chulalongkorn University from Tassanee Lerksuthirat Demo Microscope Specification Microscope: Axio Observer.Z1 / 7 Objective: Plan-Apochromat 63x/1.40 Oil DIC M27 Compression Method: Zstd1 Compression Parameters: Lossless: True Optovar: 1.25x Tubelens Filters: 200 - 405, 419 - 470, 200 - 642, 657 - 800, 200 - 488, 500 - 550, 200 - 561, 575 - 620, 200 - 405, 419 - 470, 200 - 642, 657 - 800 Imaging Device: Hamamatsu Camera Channel 1: SR HE EF BP 415-475 + LP 655 Beam Splitter: 405, 642 Filter Ex. Wavelength: 200 - 405, 200 - 642 Filter Em. Wavelength: 419 - 470, 657 - 800 Excitation Wavelength: 640 Emission Wavelength: 729 Detection Wavelength: 419-470,657-800 Channel 2: SR HE EF BP 500-550 + BP 575 Beam Splitter: 488, 561 Filter Ex. Wavelength: 200 - 488, 200 - 561 Filter Em. Wavelength: 500 - 550, 575 - 620 Excitation Wav...