SIM5 (Structured Illumination Microscopy) - Demonstrated by ZEISS
Note from the demo:
Demo Microscope Specification
Objective: Plan-Apochromat 63x/1.40 Oil DIC M27
Compression Method: Zstd1
Compression Parameters: Lossless: True
Optovar: 1.25x Tubelens
Filters: 200 - 405, 419 - 470, 200 - 642, 657 - 800, 200 - 488, 500 - 550, 200 - 561, 575 - 620, 200 - 405, 419 - 470, 200 - 642, 657 - 800
Imaging Device: Hamamatsu Camera
Channel 1: SR HE EF BP 415-475 + LP 655
Beam Splitter: 405, 642
Filter Ex. Wavelength: 200 - 405, 200 - 642
Filter Em. Wavelength: 419 - 470, 657 - 800
Excitation Wavelength: 640
Emission Wavelength: 729
Detection Wavelength: 419-470,657-800
Channel 2: SR HE EF BP 500-550 + BP 575
Beam Splitter: 488, 561
Filter Ex. Wavelength: 200 - 488, 200 - 561
Filter Em. Wavelength: 500 - 550, 575 - 620
Excitation Wavelength: 488
Emission Wavelength: 525
Detection Wavelength: 500-550,575-620
Channel 3: SR HE EF BP 415-475 + LP 655
Beam Splitter: 405, 642
Filter Ex. Wavelength: 200 - 405, 200 - 642
Filter Em. Wavelength: 419 - 470, 657 - 800
Excitation Wavelength: 405
Emission Wavelength: 445
Detection Wavelength: 419-470,657-800
Laser Wavelength: 640 nm, 488 nm, 405 nm
Algorithm: SIM2
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