Note: p38 inhibitor inhibits the apoptosis of cowanin-treated human colorectal adenocarcinoma cells
Note: p38 inhibitor inhibits the apoptosis of cowanin-treated human colorectal adenocarcinoma cells (doi: 10.3892/ijo.2018.4353) Cowanin is pure cmp. from Garcinia cowa (Chamuay); Crude extract from tree; 1. antitimor activity 2. inflmmation induction 3. antibacterial activity 4. anti-inflammatory activity 5. antimalarial activity Purpose of this study, 1. effects of cowanin on apoptosis induction 2. effect on apoptosis-related protein kinase 3.mitogen-activated protein kinase Target cell line -- LoVo human colorectal cancer cell line Assay test; 1. MTT assay (viability assay) 2. Nuclear morphological change by Hoe staining 3. Mitochondrial membrane potential by JC-1 staining Cowanin inhibits cell proliferation and induced cell dead via apoptosis pw. Cowanin induce all apoptotic related molecules through MAPK and Akt signaling pw. Therefore, the author suggested that cowanin might be a good candidate to treat colorectal cancer. T...