Short note for Tumour-associated mutations of PA-TM-RING ubiquitin ligases RNF167/RNF13 identify the PA domain as a determinant for endosomal localization
Note for myself on: Tumour-associated mutations of PA-TM-RING ubiquitin ligases RNF167/RNF13 identify the PA domain as a determinant for endosomal localization (doi: 10.1042/BJ20131067) 1. Investigate the function of RNF167/RNF13 at the cellular level -- PA domain in these two proteins were shown to function as endosomal recycling processes -- using the information from a functional study in fruitfly (homolog) and apply to the human cell 2. Using the information from COSMID to identify the mutations that could contribute to the cancer 3. Identified those mutations in human cell line// there are two separate sets; -- PA domain function --> endosomal localization -- RING domain function --> E3 ubiquitin ligase It works independently but coordinatingly to get the correct function within the cell --> right place and right time to function. Cell line; Using HEK-293 to study the function Antibodies; FK2 (recognize K29-, K48-, K63-linked poly ubiquitin...