My note on paper: A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95

Paper: A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95
(doi: 10.1016/j.yexcr.2008.01.013)

Abstract;
Facts;
-RNF43 is important for colorectal cancer
-Growth promoting factor
Gap;
-Molecular mechanism of how rnf43 promote growth is unk
Method;
-Biochemically and intracellularly characterize rnf43
Result;
-Rnf43 is in the er and nuclear envelope
-rnf43 coexists with emerin (inner nuclear membrane protein)
-rnf43 has autoubiquitination activity
-rnf43 can interact with hap95 (chromatin associated protein)
-rnf43 ubiquitinylate hap95 but only in some cell lines


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-using available database (bioexpress), rnf43 is highly expressed in colon adenoma and colon adenocarcinoma
-rnf43 is overexpressed in colorectal tumor
-evident showed when this gene is knockdown exerts growth inhibition
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Cell lines;
HeLa
COS-7
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this paper contradict to the previous report that RNF43 is secretory protein; the experiment could not be proved in this study

RNF43 interact with

*HAP95 - regulating nuclear envelop-chromatin interaction during the cell cycles

*LAP2/LAP2B - control the initiation of DNA replication

*HDAC3+AuroraB - control the mitosis process