Shomu’s Biology -- Anticancer drugs (example)

Shomu’s Biology

The peptides/protein that he mentioned in the video. If the length of the polypeptides is more than 50, we call the polypeptide a "protein".

1. Bortezomib (internal) -- MW ~ 384.237 g/mol -- targeting proteasomal pathway (peptide)

2. Nutlin (internal) -- MW ~ 581.49 g/mol -- targeting the interaction between p53-MDM2 (peptide)

3. Herceptin (external) -- MW ~ 145,531.5 g/mol -- targeting the cell surface receptor (protein)

4. Flavopiridol (internal) -- MW ~ 401.84 g/mol -- blocking the CDK to interact with cyclin partner 

5. Glevec/Tarcera (external) -- MW ~ 393.436 g/mol -- blocking the epidermal growth factor 

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I am working with Cas9 ~ MW ~ 162,200 g/mol (~163 kDa) - thus, we use electroporation to deliver the protein into the nucleus. There is no way for Cas9 to enter the cell through diffusion.