Factors to be considered for improving the HDR (IDT webinar)
Factors to be considered for improving the HDR
Improved designing
Selection of high activity guides
Using the other type of Cas – > changing from Cas9 to Cas12a
Blocking mutations to prevent re-cutting after HDR
Introducing non-complement base in gRNA to reduce the activity of Cas9
Optimize homology arm length
Same length but different gene – > give different HDR efficiency
In general sense, larger – > better HDR but it will reach plateau, thus we use the lowest length which gives the best HDR (~200 bp)
Improved reagent
Small molecule to modulate pw
Available by the company (blind solution)
Modification of repair template to increase stability
Web-based tool to design the gRNA
Info:
Comments
Post a Comment