Note for: Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40
Note for: Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40 (doi: 10.1016/j.dnarep.2004.03.039) Post-genomic era --> 1. reverse genetic tool is used to study the protein function 2. ppl using DT40 bc of many advantages; - efficient gene targeting - stable phenotype - lack p53 allow us to study the DNA damage due to the cells still can go through the interphase checkpoint even they have the error -- this is the reason why it is a good model to study the DNA damage and repair by observing on the chromosome. In higher eukaryote; 1. human and mouse, B-lymphocyte develop in bone marrow Functional IgV segments generate through site-specific process called V(D)J recombination 2. rabbit and chicken, B-lymphocyte develop in specific tissue; R-appendix of intestinal tract/C-bursa of Fabricius Functional IgV generate through intragenic homologous DNA recombination, called Ig gene conversion. Mature B-lymphocyte; hu...